10.3 The two strands of duplex DNA are complementary in sequence and arranged in antiparallel fashion. What is the base sequence of the complementary strand to the following polynucleotide: 5'-ATGCTCGACTTACAT-3' 5'-ATGTAAGTCGAGCAT-3' 5'-TACATTCAGCTCGTA-3' 3'-ATGCTCGACTTACAT-5' 5'-ATGCTCGACTTACAT-3' 5'-TACGAGCTGAATGTA-3' 10.4 Choose the false statement. The B form of DNA has 10 base pairs per turn of the helix. The melting temperature of double-stranded DNA increases as the G+C content increases. In double-stranded DNA, complementary bases are oriented toward each other on the inside of the helix. The Z form of DNA coils in a counterclockwise direction. The A form of DNA is more thin and elongated when compared to B DNA.
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Step 1: Identify the given polynucleotide sequence: 5'-ATGCTCGACTTACAT-3' Show more…
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For the DNA sequence 3'-G-C-C-T-A-T-5' in one DNA strand, the sequence found in the complementary DNA strand must be: A. 5'-C-G-G-A-T-A-3'. B. 5'-C-G-G-A-T-A-3'. C. 3'-G-C-C-T-A-T-5'. D. 5'-G-C-C-T-A-T-3'. 2. Leading and lagging DNA strands exist at a replication fork because ___________. A. the two strands of DNA are antiparallel, and DNA polymerase can only add new nucleotides onto a 3' end. B. the two strands of DNA are parallel and DNA polymerase can only add new nucleotides onto a 3' end. C. the two strands of DNA can be either parallel or antiparallel. D. the two strands of DNA are antiparallel, and DNA polymerase can only add new nucleotides onto a 5' end. 3. The enzyme responsible for removing the RNA primer during prokaryotic DNA replication is _________. A. DNA polymerase alpha. B. RNAse H. C. DNA polymerase I. 4. The enzyme Telomerase __________. A. seals the gaps between Okazaki fragments. B. removes RNA primer and replaces it with newly synthesized DNA. C. adds DNA to the end of linear chromosome.
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The segment of DNA below represents a template strand of DNA. Construct the new complementary strand of DNA and label the new strand 5' and 3' ends. 5' A T G G C C T A A T C T C C G G A 3' <--- Point of replication I I I I I I I I I I I I I I I I I New complementary strand: b. Construct the complementary parental strand above based on the parental strand in number 1 labeling the 5' and 3' ends Parental strand: (complementary) c. How does the complementary parental strand compare to the new strand you created in step number 1? d. Explain how a new strand of DNA would be constructed if the complementary parental strand was used as the template.
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Which of the following statements about DNA replication is false? a. Synthesis of the new DNA strand is from $3^{\prime}$ to $5^{\prime}$ b. Synthesis of the new DNA strand is from $5^{\prime}$ to $3^{\prime}$ c. DNA unwinds, primase adds RNA primer, and DNA polymerases synthesize the new strand and remove the RNA primer. d. Many initiation points exist in each eukaryotic chromosome. e. Okazaki fragments are synthesized in the opposite direction from the direction in which the replication fork moves.
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