103) You have an oligonucleotide probe that hybridizes to part of gene A from a eukaryotic cell. In order to carry out your work you need to make a DNA library. You have the choice of making either a genomic DNA library or a cDNA(copy DNA) library made from mRNA isolated from the cell that has been reverse transcribed back to DNA. Indicate whether a cDNA library or a genomic DNA library will be more appropriate for use in the following applications. Explain your answers. A) You discover that gene A is alternatively spliced and you want to see which predicted alternative splice products the cell actually produces. B) You want to express gene A in bacteria to produce lots of protein A.
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In this case, a **cDNA library** would be more appropriate. This is because cDNA is synthesized from mRNA, which has already undergone splicing. By analyzing the cDNA library, you can determine which splice variants are actually being produced in the cell. Show more…
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A DNA library is a collection of clones, each containing a different fragment of DNA, inserted into a cloning vector. What is the difference between a cDNA library and a genomic DNA library? Suppose you would like to clone gene $\mathrm{X},$ a gene expressed only in neurons, into a vector using a library as the source of the insert. You have the following libraries at your disposal: a genomic library from skin cells, a cDNA library from skin cells, a genomic library from neurons, and a cDNA library from neurons. Which could you use and why?
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