00:01
Which of these is not a characteristic of plasmid dna? a, they replicate independently from a bacterial chromosome.
00:08
B, they can be used in cloning.
00:10
C, they contain an origin of replication.
00:13
D, they are part of a bacterial chromosome.
00:15
E, they can contain a selectable marker.
00:19
So the incorrect statement here is d.
00:22
They are not part of the bacterial chromosome.
00:24
If i draw a big old bacterium, and let's say this here is the bacterial chromosome.
00:31
They have one chromosome and it's circular.
00:34
The plasmids are separate.
00:35
They're also circular and they are separate.
00:38
So let's say these are plasmids.
00:42
So unlike the chromosome, where you only get one copy in the cell, you can have multiple copies of the plasmids.
00:48
And this is because they do replicate independently.
00:53
And in order to be replicated, because they have their own piece of dna, they need an origin of replication.
00:59
So they are replicated just like the chromosome, so bi -directionally from the origin of replication.
01:06
So, bidirectional from the origin of replication...