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Hello everyone.
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The question here is what is the effect of concentration of substrate in enzymatic activity? so the answer to this question is that concentration of substrate is one of the factors that can affect the enzymatic activity and rate and this is done because the increase in substrate to a certain level can also increase the enzymatic activity.
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So this is the answer to this question.
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Now, we'll come to the next part.
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What are the physical and chemical requirements for the growth of microorganisms? so here in this case physical factors include factors such as the ph the temperature and the osmotic pressure whereas the chemical requirements include the presence of water and nutrients such as carbon nitrogen oxygen etc.
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Now, we'll come to the next question.
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So for the physical methods that can able to control the microbial growth.
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We can include factors such as the filtration which is a method in which we filter the microorganism.
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We can also use heat in the form of dry heat.
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For example oven and wet heat, which is autoclaving some things such as desiccation can also be used and radiation is also one such factor.
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Now, we'll come to the next question.
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The question is what are the four factors that alter or show the effectiveness of microbial treatment? so here the answer to this question is that the microbial treatment effectiveness depends upon factors such as the bacterial status then the antimicrobial concentration.
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Then we have the inoculum size serum effect and last but not the least the interaction with the host micro flora.
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Now, we'll come to the next question.
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The question is what are the factors that show the effectiveness of microbial disinfectant agent? so one of the factors include the number and location of the microorganism the concentration and potency of the disinfectants the physical and chemical factors and the resistance of microorganisms that is provided against the disinfectant.
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So these are the four factors.
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Now, the next question is what are the three methods of sterilization? so the three methods of sterilization first of all may include heat.
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So in this case, we can point out the autoclaving and oven...