16) Which of the following organisms is used to bring new genes to plants when you are creating GMOs? A. Vibrio cholerae B. adenovirus C. Bacillus thuringiensis D. Agrobacterium tumefaciens 17) When creating recombinant E. coli to express insulin, reverse transcriptase is necessary. Which of the following is the reason reverse transcriptase is used? A. It cuts the plasmid so the gene can be put in. B. It opens pores in the E. coli so the new plasmid can enter. C. It forces the E. coli to divide and express the new gene. D. It creates an intron-less gene which is essential since E. coli cannot handle introns. 18) Cancer is a result of A. overactive mitosis B. mutation C. viral infection D. All of the above are possible.
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Before a desired gene can be spliced into a bacterial plasmid cloning vector, the gene must first be isolated from the original DNA. a. cloned from the mix of recipient DNA b. injected into a stem cell to determine any unwanted traits c. injected into a bacterial cell or a virus having different sequences d. precisely cut with a restriction enzyme 6. Arrange the following events in the proper sequence for gene cloning. 1 = Fragment DNA with a restriction enzyme 2 = Isolate DNA from the organism containing the desired gene 3 = Incorporate the cloned gene into bacterial cells 4 = Incorporate the gene into a bacterial plasmid a. 1, 2, 3, 4 b. 2, 1, 4, 3 c. 4, 1, 2, 3 d. 3, 2, 1, 4 7. Plasmids are __ a. Circular pieces of DNA that are part of a bacterial chromosome. b. Separate pieces of DNA, which are not part of a bacterial chromosome. c. Contain genes for specialized functions. d. A & B e. B & C 8. Competent cells more readily take up DNA because their cell walls have been altered. a. take up DNA; cell walls b. release DNA; cell membrane c. replicate plasmids; cytoplasm d. grow and divide in log phase; cell walls
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1. Which of the following best describes Recombinant DNA? • A. DNA that has been copied into an mRNA strand. • B. A bacterial plasmid with a target gene inside it. • C. A plasmid and restriction enzyme. • D. A bacterial gene with a target plasmid inside it. 2. How does an mRNA strand reach the site for Translation? • A. It exits the Nucleus through a Nuclear Pore • B. It is already where it needs to be, Translation occurs as soon as the mRNA is processed • C. A tRNA molecule carries it to the Ribosome • D. It moves across the membrane through Active Transport 3. An organism's phenotype depends on what? • A. Genotype and Environmental factors • B. Father's DNA • C. Environmental factors • D. Genotype
9. Which of the following terms/processes could be matched with specialized transduction? a. Polyploidy b. RNA virus c. Hfr cells d. Bacterial host receives only bacterial DNA. e. Prophage 10. Which of the following statements describes Hfr cells? a. They carry an F factor plasmid that is transferred to F- cells. b. They turn an F- recipient into an F+ cell. c. They may produce genetic recombination in the recipient cell. d. They may produce genetic recombination in the donor cell. e. All of the above 11. Trisomy 21 is an example of a. polyploidy b. aneuploidy c. cytoplasmic inheritance d. deletion e. sex-linked genes 12. Individual genes may be lost in which of the following situation(s)? a. Duplication b. Translocation c. Inversion d. Deletion e. All of the above
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