How can PCR be applied to the detection of human immunodeficiency and other RNA viruses? a. The virus must be inserted into human DNA by viral integrase prior to PCR. b. Substitute deoxyuridine triphosphate in place of deoxythymidine triphosphate in the master mix. c. Add a heat-stable reverse transcriptase enzyme to the master mix. d. Substitute ribonucleotide triphosphates for deoxyribonucleotide triphosphates in the master mix.
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However, human immunodeficiency virus (HIV) and other RNA viruses have RNA as their genetic material, not DNA. To detect these RNA viruses using PCR, we need to convert their RNA into complementary DNA (cDNA) first. This process is called reverse Show more…
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