1. What is agarose? 2. What is the function of buffers in gel electrophoresis? 3. What are the charges of DNA and RNA? 4. In which direction do DNA and RNA move during electrophoresis? 5. In which direction do proteins move during electrophoresis? 6. What is the function of loading dye? 7. How do you visualize DNA on the gel? 8. What are the applications of DNA fingerprinting? 9. What are the applications of restriction endonucleases? 10. What is the application of PCR?
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Step 1: Agarose is a gel-like substance used in gel electrophoresis to separate DNA fragments based on their size. Show more…
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1- Describe the action of Agarose Gel Electrophoresis (how does the DNA run through the gel? 2- What are the three components necessary for making a viewable agarose gel?
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1. In the laboratory, describe how you would prepare a 1% agarose gel. 2. When an electric field is applied to an agarose gel in DNA gel electrophoresis, why do the DNA fragments migrate through the gel toward the anode? 3. Why is ethidium bromide or SYBR Safe added to an agarose gel? 4. What is the purpose of a DNA ladder in agarose gel electrophoresis? 5. What is the purpose of the loading dye in agarose gel electrophoresis? 6. Give an example of an appropriate loading dye. 7. Explain the purpose of UV light in agarose gel electrophoresis. 8. Why is TAE buffer used in agarose gel electrophoresis instead of water? 9. Which of the following procedures is typically used to recover the DNA sample after it has been separated by gel electrophoresis? a. A razor blade is used to cut out the DNA fragment of interest and the DNA sample within is recovered using gel purification. b. The DNA sample cannot be recovered after it has been separated by gel electrophoresis. c. A pipette is used to draw out the DNA at a select band. d. The entire gel is melted and used as the template in a PCR reaction to recover the sample of interest. 10. Give two applications of agarose gel electrophoresis.
Qbs E.
Questions $15-19$ Choose from the terms below. (A) Gel electrophoresis (B) Restriction enzymes (C) Polymerase chain reaction (D) Recombinant DNA (E) RNA processing Referred to as molecular scissors
HEREDITY
Molecular Genetics
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