2. Glyceraldehyde 3-phosphate dehydrogenase. Reaction 6 of glycolysis, the glyceraldehyde 3-phosphate dehydrogenase reaction, oxidizes and phosphorylates glyceraldehyde 3-phosphate, forming 1,3-bisphosphoglycerate. The enzyme active site sulfur atom (from a cysteine residue) and the substrate are shown below. "R" represents the rest of the enzyme.
a) A nucleophilic attack leads to the formation of a covalent tetrahedral intermediate. Highlight the atom that acts as a nucleophile and atom that acts as electrophile.