Question

2. Information about the structure of an octapeptide is given below. a. Hydrolysis of the peptide gives the following amino acids in equal amounts: Ala, Asp, Glu, Met, Lys, Phe, Tyr, and Val. b. Treatment of the peptide with 2,4-dinitrofluorobenzene followed by hydrolysis yields Asp labeled with a 2,4-DNP group. c. When the peptide is treated with carboxypeptidase, the concentration of Val increases rapidly. d. Hydrolysis of the peptide with trypsin gives a tripeptide (A) and a pentapeptide (B). e. The tripeptide (A) is cleaved by chymotrypsin to give Phe and a dipeptide with a pI of about 5.6. f. The pentapeptide (B) is cleaved by chymotrypsin to give a dipeptide and a tripeptide. Reaction of the tripeptide with cyanogen bromide gives Tyr. Write the sequence of amino acids in the octapeptide and explain how this sequence was determined.

          2. Information about the structure of an octapeptide is given below.
a. Hydrolysis of the peptide gives the following amino acids in equal amounts: Ala, Asp, Glu, Met, Lys, Phe, Tyr, and Val.
b. Treatment of the peptide with 2,4-dinitrofluorobenzene followed by hydrolysis yields Asp labeled with a 2,4-DNP group.
c. When the peptide is treated with carboxypeptidase, the concentration of Val increases rapidly.
d. Hydrolysis of the peptide with trypsin gives a tripeptide (A) and a pentapeptide (B).
e. The tripeptide (A) is cleaved by chymotrypsin to give Phe and a dipeptide with a pI of about 5.6.
f. The pentapeptide (B) is cleaved by chymotrypsin to give a dipeptide and a tripeptide. Reaction of the tripeptide with cyanogen bromide gives Tyr.
Write the sequence of amino acids in the octapeptide and explain how this sequence was determined.

2. Information about the structure of an octapeptide is given below.
a. Hydrolysis of the peptide gives the following amino acids in equal amounts: Ala, Asp, Glu, Met, Lys, Phe, Tyr, and Val.
b. Treatment of the peptide with 2,4-dinitrofluorobenzene followed by hydrolysis yields Asp labeled with a 2,4-DNP group.
c. When the peptide is treated with carboxypeptidase, the concentration of Val increases rapidly.
d. Hydrolysis of the peptide with trypsin gives a tripeptide (A) and a pentapeptide (B).
e. The tripeptide (A) is cleaved by chymotrypsin to give Phe and a dipeptide with a pI of about 5.6.
f. The pentapeptide (B) is cleaved by chymotrypsin to give a dipeptide and a tripeptide. Reaction of the tripeptide with cyanogen bromide gives Tyr.
Write the sequence of amino acids in the octapeptide and explain how this sequence was determined.

Added by Francisca K.

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