24 Multiple Choice 2 points To map genes of a bacterial strain, conjugation must be interrupted at give and you combine these two cultures in liquid medium in four blenders at t blender play in the experiment? speeds up molecular movement of DNA shears the sex pili of conjugants hastens conjugation events serves as a culture container only acts as a mechanism to bring cells into contact
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E. Can't tell 53. Approximately how far apart is the Lac gene from the Phe Gene? A. 5 minutes B. 10 minutes C. 15 minutes D. More than 25 minutes E. Can't tell 54. Approximately how far apart is the Gal gene from the Trp Gene A. 5 minutes B. 10 minutes C. 15 minutes D. 7 minutes E. More than 15 minutes 55. What is the key benefit of a temperature sensitive mutant screen over a mutant selection? A. Can find natural variants present in the environment B. Can find mutants defective in essential biological processes C. Can use this to study bacterial ecology D. Kill wildtype cells to obtain only mutants E. You know what genes you will find. 56. After doing the mating, which gene has had no genetic use so far? A. Str B. Kan C. Arg D. Phe E. Trp 57. What is the major capacity that liquid media can accomplish to facilitate mutant screens that is not possible in solid media? A. Separate cells from each other B. Grow up large populations in one container C. Grow bacteria and screen for phenotypes D. Grow bacteria and select for phenotypes E. Make master plates.
Adi S.
1. How long does it take to obtain an OD600 measurement with a spectrophotometer? A. 24 hours B. 1 hour C. It is instantaneous D. It depends on the density of bacteria 2. What does it mean to "vortex?" A. Mix B. Incubate C. Spread D. Separate
Suman K.
Quiz #4: Bacterial Gene Mapping In an interrupted mating experiment between an E. coli Hfr strain with the genotype a+b+c+d+e+f+ and an F- auxotrophic strain with the genotype a-b-c-d-e-f-, conjugation was stopped at intervals of 10 minutes, and the genotypes of the conjugates were determined. The following results were obtained at each time interval: 10 minutes: e+ 20 minutes: a+e+ 30 minutes: a+b+e+ 40 minutes: a+b+d+e+ 50 minutes: a+b+c+d+e+ 60 minutes: a+b+c+d+e+f+ Please refer to the table below for the results obtained: Time Interval Genotype 10 minutes e+ 20 minutes a+e+ 30 minutes a+b+e+ 40 minutes a+b+d+e+ 50 minutes a+b+c+d+e+ 60 minutes a+b+c+d+e+f+ Using this data, we can establish the order of genes on a bacterial chromosome and determine the point of origin.
Jenny W.
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