00:01
Hello everyone in this question we will be talking about pcr which stands for polymerase chain reaction.
00:10
In this the in vitro replication is taking place.
00:16
The components of the pcr are buffer dn -t primers, stack dna polymer, dna, dna template and magnetion ion as a co -factor for enzymes.
00:34
So in this the first step is the denaturation step, which takes place at the 90 degrees celsius.
00:46
In this, the double -stranded dna, which are hydrogen bonded from each other, are denatured, that is, the hydrogen bond here are, broken down due to the high temperature and after this we will have two strands of dna.
01:11
So this avoids the activity of helicase that is present in the in vivo dna replication.
01:22
So here we have two single stranded dna now.
01:26
Then the next step is the annealing step, which is also known as as the primer anulium step.
01:36
So here we have two strands of dna...