3. Differentiate between alpha- and beta-hemolysis. 4. Describe the appearance of S. aureus on Blood Agar. 5. Why is the coagulase test considered to be the definitive test for S. aureus? (refer to Ex. 53) 6. What is the role of coagulase in the pathogenesis of S. aureus? (refer to Ex. 53) 7. Why are the Staphylococci among the leading causes of healthcare-associated infections? (refer to Ex. 53)
Added by Luisa R.
Close
Step 1
Differentiate between alpha- and beta-hemolysis. Alpha-hemolysis: Partial lysis of red blood cells, resulting in a greenish or brownish discoloration around the colonies. Beta-hemolysis: Complete lysis of red blood cells, resulting in a clear zone around the Show more…
Show all steps
Your feedback will help us improve your experience
Sri K and 95 other Biology educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
What is hemolysis and what medium is used to demonstrate hemolysis? Distinguish between alpha-, beta-, gamma-, and double zone hemolysis. What is the substrate for the coagulase test? What is a selective medium and differential medium? Among the Staphylococci, which enzyme production correlates most closely with pathogenicity? How can a Streptococci isolate be distinguished from a Staphylococci isolate? How can a pigmented saprophyticus isolate be distinguished from aureus?
Sri K.
What is the result of Staphylococcus epidermidis on MSA? Describe how you came to this conclusion. What are the types of hemolysis that these Gram + bacteria show? Describe what the types mean the bacteria can or cannot do. What is the result of Staphylococcus epidermidis on blood agar? Describe how you came to this conclusion. What is the result of Staphylococcus epidermidis in the catalase test? What does this mean the bacteria does not contain? What is the result of Staphylococcus aureus? What does this mean the bacteria does not contain? Decomposition Coagulase test) What does this mean the bacteria does not contain? Review the overall data for the bacteria (Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus). If you just do a Gram stain on these bacteria, could you determine which bacteria you had? Why or why not?
Adi S.
3. How can the genera Staphylococcus and Micrococcus be differentiated from each other? 4. Which enzyme that can be detected in the clinical laboratory is most closely correlated with the pathogenicity of Staphylococcus aureus? 5. Explain the principle of the catalase test. What product is detected when the catalase test is positive? 6. How could a strain of Staphylococcus aureus be slide coagulase-negative and tube coagulase positive?
Madhur L.
Recommended Textbooks
Biology for AP Courses
Objective Biology for NEET
Introduction to General, Organic and Biochemistry
Transcript
18,000,000+
Students on Numerade
Trusted by students at 8,000+ universities
Watch the video solution with this free unlock.
EMAIL
PASSWORD