3. DNA has a negative charge. It reacts with a positive ion in solution to precipitate in the ethanol. Where is the positive ion introduced?
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3 What is the purpose for filtering the mixture through the cheesecloth OR centrifugation? DNA molecules are negatively charged. How do water molecules interact with negative ions? Is DNA soluble in water? 5 What ions are created when table salt (NaCl) are placed into water? Which ion would bind to the negative phosphate charges found in DNA? 9 After the salt binds to the DNA and neutralizes all of the DNA charges, and alcohol causes the DNA molecules to aggregate, this causes DNA to out of the ice-cold alcohol solution.
Sri K.
describe the structure of DNA and explain why DNA is negatively-charged. 4. the action of detergent on membranes: what does it do, and how? 5. the precipitation of DNA: explain the role of sodium ions - what do they do to DNA molecules, and why does this cause the precipitation of DNA? 6. the action of the alcohol: how does this enhance the binding of sodium ions to the DNA? 7. explain why the precipitated DNA floats, rather than sinking
Maryam R.
In isolation of genetic material (DNA) from nucleus, the RNA can be removed by ribonuclease and protein by protease. The purified DNA then precipitates after the addition of (a) $\mathrm{CaCl}_{2}$ (b) Ether (c) Chilled ethanol (d) Acetic acid
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