Question

36. A research scientist carries out the following procedure: Step 1: Plasmids are generated using restriction enzymes and DNA ligase. The plasmids include these segments of DNA: a promoter, an ampicillin resistance gene, and a gene coding for human insulin. Step 2: The plasmids created in step 1 are introduced into E. coli cells by transformation. Step 3: The transformed cells are cultured in a nutritionally rich growth medium containing the antibiotic ampicillin. What should the scientist observe after completing step 3?: (A) There will be no E. coli cells remaining because E. coli cells are not naturally resistant to ampicillin. (B) There will be no E. coli cells remaining because transformation causes cell lysis. (C) The remaining E. coli cells will contain insulin mRNA and insulin protein. (D) The remaining cells will contain insulin receptor protein and insulin protein. 37. What role does rRNA play in the cell?: (A) rRNA encodes the messages for genes that will be translated by ribosomes in the cytoplasm. (B) rRNA anticodons pair with mRNA codons, bringing the correct amino acid into position to be added to the peptide. (C) rRNA is the nucleic acid component of the ribosome; it binds RNA messages during translation. (D) rRNA encodes a nuclear gene in a cell prior to transcription by host polymerases.

          36. A research scientist carries out the following procedure:

Step 1: Plasmids are generated using restriction enzymes and DNA ligase. The plasmids include these segments of DNA: a promoter, an ampicillin resistance gene, and a gene coding for human insulin.
Step 2: The plasmids created in step 1 are introduced into E. coli cells by transformation.
Step 3: The transformed cells are cultured in a nutritionally rich growth medium containing the antibiotic ampicillin.

What should the scientist observe after completing step 3?:
(A) There will be no E. coli cells remaining because E. coli cells are not naturally resistant to ampicillin.
(B) There will be no E. coli cells remaining because transformation causes cell lysis.
(C) The remaining E. coli cells will contain insulin mRNA and insulin protein.
(D) The remaining cells will contain insulin receptor protein and insulin protein.

37. What role does rRNA play in the cell?:
(A) rRNA encodes the messages for genes that will be translated by ribosomes in the cytoplasm.
(B) rRNA anticodons pair with mRNA codons, bringing the correct amino acid into position to be added to the peptide.
(C) rRNA is the nucleic acid component of the ribosome; it binds RNA messages during translation.
(D) rRNA encodes a nuclear gene in a cell prior to transcription by host polymerases.

36. A research scientist carries out the following procedure:

Step 1: Plasmids are generated using restriction enzymes and DNA ligase. The plasmids include these segments of DNA: a promoter, an ampicillin resistance gene, and a gene coding for human insulin.
Step 2: The plasmids created in step 1 are introduced into E. coli cells by transformation.
Step 3: The transformed cells are cultured in a nutritionally rich growth medium containing the antibiotic ampicillin.

What should the scientist observe after completing step 3?:
(A) There will be no E. coli cells remaining because E. coli cells are not naturally resistant to ampicillin.
(B) There will be no E. coli cells remaining because transformation causes cell lysis.
(C) The remaining E. coli cells will contain insulin mRNA and insulin protein.
(D) The remaining cells will contain insulin receptor protein and insulin protein.

37. What role does rRNA play in the cell?:
(A) rRNA encodes the messages for genes that will be translated by ribosomes in the cytoplasm.
(B) rRNA anticodons pair with mRNA codons, bringing the correct amino acid into position to be added to the peptide.
(C) rRNA is the nucleic acid component of the ribosome; it binds RNA messages during translation.
(D) rRNA encodes a nuclear gene in a cell prior to transcription by host polymerases.

Added by Brenda T.

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