Question

3.6. During a test of kinetics of an enzyme-catalyzed reaction, the following data were recorded: E? (g/l) T (°C) I (mmol/ml) S (mmol/ml) V (mmol/ml-min) 1.6 30 0 0.1 2.63 1.6 30 0 0.033 1.92 1.6 30 0 0.02 1.47 1.6 30 0 0.01 0.96 1.6 30 0 0.005 0.56 1.6 49.6 0 0.1 5.13 1.6 49.6 0 0.033 3.70 1.6 49.6 0 0.01 1.89 1.6 49.6 0 0.0067 1.43 1.6 49.6 0 0.005 1.11 0.92 30 0 0.1 1.64 0.92 30 0 0.02 0.90 0.92 30 0 0.01 0.58 0.92 30 0.6 0.1 1.33 0.92 30 0.6 0.033 0.80 0.92 30 0.6 0.02 0.57 a. Determine the Michaelis–Menten constant for the reaction with no inhibitor present at 30°C and at 49.6°C. b. Determine the maximum velocity of the uninhibited reaction at 30°C and an enzyme concentration of 1.6 g/l. c. Determine the K? for the inhibitor at 30°C and decide what type of inhibitor is being used.

          3.6. During a test of kinetics of an enzyme-catalyzed reaction, the following data were recorded:

E? (g/l) T (°C) I (mmol/ml) S (mmol/ml) V (mmol/ml-min)
1.6 30 0 0.1 2.63
1.6 30 0 0.033 1.92
1.6 30 0 0.02 1.47
1.6 30 0 0.01 0.96
1.6 30 0 0.005 0.56
1.6 49.6 0 0.1 5.13
1.6 49.6 0 0.033 3.70
1.6 49.6 0 0.01 1.89
1.6 49.6 0 0.0067 1.43
1.6 49.6 0 0.005 1.11
0.92 30 0 0.1 1.64
0.92 30 0 0.02 0.90
0.92 30 0 0.01 0.58
0.92 30 0.6 0.1 1.33
0.92 30 0.6 0.033 0.80
0.92 30 0.6 0.02 0.57

a. Determine the Michaelis–Menten constant for the reaction with no inhibitor present at 30°C and at 49.6°C.
b. Determine the maximum velocity of the uninhibited reaction at 30°C and an enzyme concentration of 1.6 g/l.
c. Determine the K? for the inhibitor at 30°C and decide what type of inhibitor is being used.

3.6. During a test of kinetics of an enzyme-catalyzed reaction, the following data were recorded:

E? (g/l) T (°C) I (mmol/ml) S (mmol/ml) V (mmol/ml-min)
1.6 30 0 0.1 2.63
1.6 30 0 0.033 1.92
1.6 30 0 0.02 1.47
1.6 30 0 0.01 0.96
1.6 30 0 0.005 0.56
1.6 49.6 0 0.1 5.13
1.6 49.6 0 0.033 3.70
1.6 49.6 0 0.01 1.89
1.6 49.6 0 0.0067 1.43
1.6 49.6 0 0.005 1.11
0.92 30 0 0.1 1.64
0.92 30 0 0.02 0.90
0.92 30 0 0.01 0.58
0.92 30 0.6 0.1 1.33
0.92 30 0.6 0.033 0.80
0.92 30 0.6 0.02 0.57

a. Determine the Michaelis–Menten constant for the reaction with no inhibitor present at 30°C and at 49.6°C.
b. Determine the maximum velocity of the uninhibited reaction at 30°C and an enzyme concentration of 1.6 g/l.
c. Determine the K? for the inhibitor at 30°C and decide what type of inhibitor is being used.

Added by Andrea M.

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