4) Analysis of unordered yeast tetrads from the cross ade ura? x ade? ura yielded the following data: Tetrad class Spore Phenotypes # of Asci 1 ade ura? ade ura? ade? ura ade? ura 56 2 ade ura? ade ura ade? ura? ade? ura 40 3 ade ura ade ura ade? ura? ade? ura? 2 What is the map distance between the genes? a. The genes are not linked b. 20.4% c. 42.9% d. 40.8% e. 22.4%
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Identify the tetrad class. The tetrad class is ade ura ade ura ade ura. Show moreā¦
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J24: A diploid yeast strain of the genotype his4 LEU2 / HIS4 leu2 is induced to undergo meiosis and the resulting spore colonies are scored for their ability to grow in the absence of histidine or leucine. The following results were obtained for 443 tetrads: PD: 282 NPD: 3 T: 158. What is the map distance in centimorgans (cM) between his4 and leu2?
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(a) The diploid yeast genome is 2.8 x 10^7 base pairs. The diploid human genome is 6.2 x 10^9 bp. If all eukaryotes were identical in gene density, and the average gene size was 2,000 bp and there was no intergenic space, how many genes would you predict for each organism? Is that close to the realities for these organisms? (b) Gene density is not the same for most organisms. What yeast- and human-specific features contribute to deviation from the expected?
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A yeast geneticist irradiates haploid cells of a strain that is an adenine-requiring auxotrophic mutant, caused by mutation of the gene ade1. Millions of the irradiated cells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies. These colonies are crossed individually with a wildtype strain. Two types of results are obtained: (1) prototroph $\times$ wild type : progeny all prototrophic (2) prototroph $\times$ wild type : progeny $75 \%$ prototrophic, $25 \%$ adenine-requiring auxotrophs a. Explain the difference between these two types of results. b. Write the genotypes of the prototrophs in each case. c. What progeny phenotypes and ratios do you predict from crossing a prototroph of type 2 by the original ade1 auxotroph?
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