4) Look up the function of your protein using GOOGLE and Web of Science (the latter from on campus or using a VPN). a) What does your protein do? b) Give me the reference to a recent review article about your protein.
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Part 2: Identify one or more review articles (or primary literature articles) about your cell part. You may use either PubMed, Google Scholar, Science Direct, and/or Web of Knowledge. If you have trouble finding articles about your cell part, be sure that you search using multiple different search terms and in multiple different places. For example, if you were assigned Nucleus, you might search for "Nucleus", "Nuclear trafficking", "Nuclear Lamina", "Nuclear Pore complex", etc. When using PubMed, be sure to check 'review' on the left to search for review articles at first. For other databases, try typing "review" in the search bar. After you are familiar with the current state of the field, you can check out some primary literature articles (non-review articles). 1. Using review and primary literature articles, try to answer the questions you posed above (in part 1 question 2). What things did you learn in your research that you didn't know before? 2. What is still unknown about your cell part? In other words, what questions are scientists still trying to answer about it? 3. How does your cell part relate to human or wildlife disease? 4. List your scientific primary and review journal article(s) that you used for your answers here (Include the last name of authors, title of the article, journal it was published in, and the year it was published - don't worry about formatting your references)
Suman K.
I am a third year PhD student with a 120 KDa PAS domain protein that is not crystallising. I can purify it in milligram quantities to a very pure state. I am not sure why it is not crystallising. It is a large protein of which some of the domains, but not all, have been solved previously by X-ray crystallography. 1) What two (2) alternative experimental techniques can I use to characterise my protein? [2 min] 2) Briefly describe the biophysical basis for these 2 techniques. [4 min] 3) Name one (1) possible outcome for each experiment and how this could help in your quest to obtain protein crystals.
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Researchers use a variety of techniques to learn more about the function of a specific gene in an organism. In one type of experiment, called a loss-of-function experiment, the gene being investigated is eliminated. In a gain-of-function experiment, extra copies of the gene being investigated are inserted. The cell process most directly affected in both experiments is (1) protein synthesis (2) waste disposal (3) transport of materials (4) breakdown of nutrients
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