4.2
(a) The distances travelled by four compounds and the solvent front after a TLC separation on a polar silica plate were as follows:
Compound Distance travelled
Solvent 7.5 cm
A 4.2 cm
B 3.5 cm
C 2.3 cm
D 2.3 cm
Calculate the $R_f$ value of each compound, clearly draw the TLC results and comment on the polarity of each compound.
(b) Roughly draw a GC chromatogram for the TLC results in (a) above.
4.3 Discuss the basic principles of GC, HPLC and TLC.
4.4 Differentiate between planar and column chromatographic methods.