Question

5. (a) This question deals with thin layer chromatography (TLC) (review Lab #7 and Lab #8). The TLC plates we use are coated with silica gel. Using a nonpolar solvent in the chamber which will move further up the TLC plate, a polar organic molecule or a nonpolar organic molecule, and explain your answer? [2 marks] (b) A sample run on a TLC plate migrated 10 mm from the initial spot, while the solvent front ran 15 mm total. What was the Rf value of the spot? Show your work. [1 mark] (c) If we wanted the spot to migrate further on the silica plate, what could we do to facilitate the sample to run further on the TLC plate, other than leaving the reaction to proceed longer in the chamber? [1 mark]

          5. (a) This question deals with thin layer chromatography (TLC) (review Lab #7 and Lab #8). The TLC plates we use are coated with silica gel. Using a nonpolar solvent in the chamber which will move further up the TLC plate, a polar organic molecule or a nonpolar organic molecule, and explain your answer? [2 marks]

(b) A sample run on a TLC plate migrated 10 mm from the initial spot, while the solvent front ran 15 mm total. What was the Rf value of the spot? Show your work. [1 mark]

(c) If we wanted the spot to migrate further on the silica plate, what could we do to facilitate the sample to run further on the TLC plate, other than leaving the reaction to proceed longer in the chamber? [1 mark]

5. (a) This question deals with thin layer chromatography (TLC) (review Lab #7 and Lab #8). The TLC plates we use are coated with silica gel. Using a nonpolar solvent in the chamber which will move further up the TLC plate, a polar organic molecule or a nonpolar organic molecule, and explain your answer? [2 marks]

(b) A sample run on a TLC plate migrated 10 mm from the initial spot, while the solvent front ran 15 mm total. What was the Rf value of the spot? Show your work. [1 mark]

(c) If we wanted the spot to migrate further on the silica plate, what could we do to facilitate the sample to run further on the TLC plate, other than leaving the reaction to proceed longer in the chamber? [1 mark]

Added by Natalie J.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Sulav Pokhrel

10/18/2022

Step 1

Since the TLC plates are coated with silica gel, which is polar, a nonpolar solvent will interact more with nonpolar organic molecules. Therefore, the nonpolar organic molecule will move further up the TLC plate compared to a polar organic molecule. ** Show more…

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(a) This question deals with thin layer chromatography (TLC) (review Lab #7 and Lab #8). The TLC plates we use are coated with silica gel. Using a nonpolar solvent in the chamber, which will move further up the TLC plate: a polar organic molecule or a nonpolar organic molecule? Explain your answer. [2 marks] (b) A sample run on a TLC plate migrated 10 mm from the initial spot, while the solvent front ran 15 mm in total. What was the Rf value of the spot? Show your work. [1 mark] (c) If we wanted the spot to migrate further on the silica plate, what could we do to facilitate the sample to run further on the TLC plate, other than leaving the reaction to proceed longer in the chamber? [1 mark]