5- Haemophilus influenzae will not normally grow on 5% sheep blood agar. However, the organism will grow as satellite colonies around the or Staphylococcus aureus on BAP. What is the reason for this? A-Heamophilus influenzae organisms like to grow near Staphylococcus aureus B-BAP does not contain hemin or NAD but Staphylococcus aureus produces both hemi and NAD C-BAP contains NAD and Staphylococcus aureus produces hemin D-BAP contains hemin and Staphylococcus aureus produces NAD
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Step 1: Identify the nutritional requirements of Haemophilus influenzae — it requires X factor (hemin) and V factor (NAD) to grow. Show more…
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Haemophilus, influenzae grows best at $35-37^{\circ} \mathrm{C}$ with $\sim 5 \% \mathrm{CO}_{2}$ (or in a candle-jar) and requires hemin (X factor) and nicotinamide-adenine-dinucleotide (NAD, also known as V factor) for growth.[2] Using the vocabulary learned in this chapter, describe $H$. influenzae.
Incomplete lysis of sheep red blood cells with the production of methemoglobin products, giving rise to a clear zone around bacteria colonies. Bacillus subtilis shows no zone around the bacteria colonies. Escherichia coli shows a greenish zone around the bacteria colonies. Streptococcus mutans exhibits gamma hemolysis. Staphylococcus epidermis does not show any of the above characteristics. A recombinant strain of Pseudomonas syringae, developed using recombinant DNA technology, characteristically does not ferment sugars. Therefore, this organism appears as red/pink or purplish colonies on EMB agar, colorless colonies on MacConkey's agar, and tiny pinpoint colonies on blood agar. It only grows in the bottom of thioglycolate media. The mucoid characteristic of a bacteria colony is typically associated with bacteria such as Proteus vulgaris, which produces flagella. It is not associated with cell wall production in Corynebacterium species, catalase production in Staphylococcus species, or capsule production in Klebsiella species. Some bacteria, such as pneumonae and Klebsiella species, protect themselves against the humoral immune response and/or the inflammatory response in an infected host by producing a slime layer. This protection is not achieved through cell wall production in Streptococcus species, pili production in Streptococcus species, or capsule production in any of the above species. Streptokinase, an enzyme used in cardiac therapy, is produced by gram-positive cocci bacteria. It is not produced by bacilli, gram-negative cocci, or gram-negative microorganisms. Shigella species on TSI agar produces a red slant and a yellow butt, indicating fermentation of glucose and lactose. It does not ferment sucrose.
Adi S.
7. Why do we use Staphylococcus aureus when performing the CAMP test?
Jennifer S.
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