Question

5 On a given microscope, the numerical apertures of the condenser and low-power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520 nm. a. What is the limit of resolution on this microscope? 520 / (1.25 + 0.25) = 347 nm b. Will you be able to distinguish two points that are 300 nm apart as being separate, or will they blur into one? MICROBIOLOGY: Laboratory Theory & Application, Brief

          5 On a given microscope, the numerical apertures of the condenser and low-power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520 nm.
a. What is the limit of resolution on this microscope?
520 / (1.25 + 0.25) = 347 nm
b. Will you be able to distinguish two points that are 300 nm apart as being separate, or will they blur into one?
MICROBIOLOGY: Laboratory Theory & Application, Brief

5 On a given microscope, the numerical apertures of the condenser and low-power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520 nm.
a. What is the limit of resolution on this microscope?
520 / (1.25 + 0.25) = 347 nm
b. Will you be able to distinguish two points that are 300 nm apart as being separate, or will they blur into one?
MICROBIOLOGY: Laboratory Theory Application, Brief

Added by Jose Angel C.

Biology for AP Courses

Julianne Zedalis, John Eggebrecht

Instant Answer

Solved by Expert Adi S

06/27/2023

Step 1

The limit of resolution (d) can be calculated using the Abbe's formula: d = \frac{\lambda}{2 \times NA} where λ is the wavelength of light and NA is the numerical aperture. Show more…

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On a given microscope, the numerical apertures of the condenser and low-power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520 nm. a. What is the limit of resolution on this microscope? b. Will you be able to distinguish two points that are 300 nm apart as being separate, or will they blur into one?