Question

5) The following sequence of bases is present along one chain of a DNA double-helix that has opened up at a replication fork, and synthesis of an RNA primer on this template begins by copying the base in bold. 3' - ... TCT GAT ATC AGT ACG ... - 5' a) If the RNA primer consists of 8 nucleotides, what is its base sequence? b) In the intact RNA primer, which nucleotide has a free hydroxyl (-OH) terminus and what is the chemical group on the nucleotide at the other end of the primer? c) If replication of the other strand of the original DNA double-helix proceeds continuously (with few or no intervening RNA primers), is the replication fork more likely to move in a left-to-right or a right-to-left direction? d) Based on your answer to c), where would helicase be most likely located? e) Which strand would ligase be closer to, the one listed above or its complementary strand? Explain why.

          5) The following sequence of bases is present along one chain of
a DNA double-helix that has opened up at a replication fork, and
synthesis of an RNA primer on this template begins by copying the
base in bold. 3' - ... TCT GAT ATC AGT ACG ... -
5'
a) If the RNA primer consists of 8 nucleotides, what is its base
sequence?
b) In the intact RNA primer, which nucleotide has a free
hydroxyl (-OH) terminus and what is the chemical group on the
nucleotide at the other end of the primer?
c) If replication of the other strand of the original DNA
double-helix proceeds continuously (with few or no intervening RNA
primers), is the replication fork more likely to move in a
left-to-right or a right-to-left direction?
d) Based on your answer to c), where would helicase be most
likely located?
e) Which strand would ligase be closer to, the one listed above
or its complementary strand? Explain why.

Added by Leslie R.

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