00:01
So this question is looking at two different protein complexes, two different subunits, and running them on different types of gel.
00:09
The first gel is an fds page, and the second gel is a native page.
00:15
So we have our sds page and our native page.
00:28
Okay, so for this first question, it says that there is a protein with four subunits.
00:32
So i'm going to draw those out here.
00:33
We have one, two, three, and four.
00:40
And it says that three of those subunits are 35 kilidaltons, and that one of those subunits is 110 kilotalton.
01:03
And it's asking us how many bands we would see on this sds page gel.
01:08
So for sds page, sds denatures the proteins.
01:13
This means it's going to linearize all of the proteins, and it's also going to break apart these interactions between different subunits...