5ggcatgcgaaa3 3ccgtacgcttt5 a how many strands of dna are...

5’GGCATGCGAAA3’ 3’CCGTACGCTTT5’ A. How many strands of DNA are present? B. How many hydrogen bonds should be found between the strands? C. How many phosphodiester bonds would be present in total? D. If the molecule was circular, how many hydrogen bonds would be present? E. If the molecule was circular, how many phosphodiester bonds would be present?

Transcript

00:01 Hello everyone, for this question we have to answer two parts starting with question number 2 given.

00:05 So here we are given 3000 base pairs in a plasmid.

00:15 So for this the number of nucleotides will be equal to 6000 that is twice the number of base pairs.

00:22 Now starting with the a part where we have to find the phosphodiester bonds, they will be equal to 6000 -1 as one phosphodiester bond is present in each nucleotide.

00:40 So this equals to 5999.

00:47 Then for b the number of n -glycosidic bonds, they will be equal to the number of nucleotides that is 6000 as one nucleotide contains one n -glycosidic bond.

01:03 And for c the number of hydrogen bonds, so as given the g plus c content is equal to 50%, this shows that g equal to c equals to 3000 divided by 2 which equal to 1500.

01:28 So number of hydrogen bonds in g triple bond c will be 1500 multiplied by 3 that is 4500 whereas for adenine and thymine there are two hydrogen bonds so it will be 1500 multiplied by 2 which is equal to 3000.

01:54 So this equals the total hydrogen bonds will be 7500.

02:03 Next ask is question number e for which the number of purines are to be determined.

02:08 So purines is equal to adenine and guanine are the purines and they both are 750 which equals to 1500 purines.

02:25 For f the number of phosphorus atoms, so phosphorus atoms they are equal to 1 per nucleotide.

02:44 So 6000 nucleotides will contain 6000 phosphorus atoms.

02:50 Then further the number of nitrogen atom for the g part.

03:01 So nitrogen atoms if we talk about the purines they have 10 nitrogen atoms while the pyrimidines have 5.

03:17 So we have the number of purines 1500 so 10 into 1500 equals to 15000 while 10 or this is 5 into 1500 equals to 7500.

03:41 So total number of hydrogen atoms they will be equal to 32500.

03:51 Similarly for question number 3 given we are given the plasmid with 1000 base pairs.

04:02 So the nucleotides will be equal to 2000.

04:05 Now for the a part phosphodiester bonds just like the above case they will be equal to 2000 minus 1 that is equal to 1999...

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