8. (8 pts) Match the following enzymes/proteins/structures to its correct function: A. AUG B. DNA ligase C. DNA polymerase D. Helicase E. Promoter F. RNA polymerase G. Terminator H. Topoisomerase ______ Makes DNA from a DNA template ______ Start codon ______ Makes RNA from a DNA template ______ Signals start of transcription ______ Connects Okazaki fragments ______ Signals end of transcription ______ Relaxes tension in the upstream DNA ______ Unwinds DNA 9. (1 pt) Dr. Nerdman wants to inhibit a cell from producing Protein A. Which of the following experimental treatments could he use to accomplish his goal? a) Expose the cell to a chemical that binds to the promoter of the gene that encodes Protein A. b) Expose the cell to a chemical that inhibits the large and small ribosomal subunits from binding. c) Expose the cell to a chemical that degrades all mRNA. d) Expose the cell to a chemical that degrades all tRNA. e) All of the above.
Added by Derrick R.
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This would prevent the gene from being transcribed into mRNA, and thus, Protein A would not be produced. Show more…
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Match the proteins/ enzymes below with their functions (a-e) 1. Single-stranded binding proteins 2. Ligase 3. Gyrase (Topoisomerase) 4. Helicase 5. Primase a. Initiates unwinding of double-stranded DNA by separating parental strands (breaks hydrogen bonds) b. Connects two separate fragments of DNA by creating a phosphodiester bond between them. c. eliminates the stress caused by unwinding of double-stranded DNA (relieves torsional tension) d. synthesizes a short RNA primer that is complementary to a region of template strand. e. bind to single stranded DNA and prevent the duplex (double stranded DNA) from reforming.
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Match the enzyme or protein with the correct description of its activity in bacterial DNA replication. Chains most nucleotides together during DNA replication DNA gyrase or Topoisomerase DNA Polymerase III DNA Helicase Single stranded binding proteins DNA Polymerase I DNA Primase DNA Polymerase II RNA Polymerase Sits in the replication fork while breaking hydrogen bonds between base pairs DNA gyrase or Topoisomerase DNA Polymerase III DNA Helicase Single stranded binding proteins DNA Polymerase I DNA Primase DNA Polymerase II RNA Polymerase Functions ahead of the replication fork to relieve excess supercoiling as the DNA opens up DNA gyrase or Topoisomerase DNA Polymerase III DNA Helicase Single stranded binding proteins DNA Polymerase I DNA Primase DNA Polymerase II RNA Polymerase Coats the single stranded regions of DNA so they don't base pair internally
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