A biochemist wants to use CHO cells to scale up production of the mouse CB2 (mCB2) receptor. Which components of the recombinant gene should come from the mouse and which should come from the CHO cells? CHO regulatory sequence attached to the mouse CB2 coding sequence mouse chromosome injected into the CHO cell CHO CB2 coding sequence attached to the mouse regulatory sequence mouse regulatory sequence and mCB2 coding sequence
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This requires creating a recombinant gene that will be expressed in the CHO cells. Show more…
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You synthesized an enhancerless GFP reporter gene in which the GFP cDNA is placed downstream of a promoter that functions in mice. You have fused this enhancerless GFP reporter to fragments of genomic DNA surrounding the transcribed part of a mouse gene. Each reporter construct is introduced into the nucleus of a fertilized mouse egg (zygote) to make transgenic mice. You analyze the pattern of GFP expression in these mice and obtain the following results: Genomic DNA surrounding a mouse gene GFP expression pattern 1 2 3 GFP Eye and Leg GFP Eye GFP Leg GFP Eye and Leg PAX6 gene regulates development of the eye across many species. You want to test if the human PAX6 protein can substitute the mouse PAX6 protein in eye development. What sequence do you need to fuse to the human PAX6 cDNA to cause PAX6 expression in mouse eyes? Mouse promoter Mouse promoter and region 3 Mouse promoter and region 2 Random mouse genome sequences Mouse promoter and region 1 None because human regulatory sequences are needed.
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CHO cell culture is used to make the antibody. CHO cells stands for Chinese hamster ovary cells. They are not immune cells (B cells). So why can CHO cells be used to produce antibody? Also describe the configuration of a suitable bioreactor for cultivating CHO fermentation. Justify your choice.
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