a) Define Km. Using the Michaelis-Menten equation, solve Km when V0 is exactly one-half of Vmax. [Here, V0 is the initial velocity, and Vmax is the maximum velocity.] [2.5]
b) Considering the interaction of 2, 3-bisphosphoglycerate (BPG) with hemoglobin molecules, what kind of relationship exists between BPG and oxygen binding in a cellular environment? Explain. [2.5 points]