A doctor from Overlake Hospital knows that you have been working with bovine pancreatic cells. He wants you to take a look at the proteins expressed in the cells of the pancreas of a 54 year old patient that has a pancreatic tumor. What method will you use to roughly estimate how many proteins are expressed in those tumor cells? Hint: there are lots and lots of proteins. Hint: you can't do it by just running your sample on a gel.
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To correctly perform and interpret proteomic analysis, careful attention to the following may be needed: - Proteins' compositions can be influenced by pH, hypoxia, anesthesia treatment, or other environments, which may influence the outcome of an experiment. - The myriad ways proteins can be processed and modified. - The large range of protein abundances observed, spanning 10 to the power of 10 orders of magnitude in eukaryotes. - Selecting among the numerous PCR protocols for amplifying the naturally observed proteins.
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