Question

A five fold serial dilution is performed up to 5 tubes. Explain the steps in detail on how to perform the serial dilution. Have plenty of sample to work with. Indicate size of pipettes will be using and diluent. Sample is serum. Part B: Give the tube dilution in each tube and the solution dilution in each tube. If the original sample was 550 mg/dL what is the diluted result in tube #4. 

          A five fold serial dilution is performed up to 5 tubes.  Explain the steps in detail on how to perform the serial dilution.   Have plenty of sample to work with.  Indicate size of pipettes will be using and diluent.
Sample is serum.
Part B:
Give the tube dilution in each tube and the solution dilution in each tube.  If the original sample was 550 mg/dL what is the diluted result in tube #4.
Show more…

Added by Juan Luis L.

Biology for AP Courses
Biology for AP Courses
Julianne Zedalis, John Eggebrecht
AceChat toggle button
Close icon
Ace pointing down

Please give Ace some feedback

Your feedback will help us improve your experience

Thumb up icon Thumb down icon
Thanks for your feedback!
Profile picture
A five fold serial dilution is performed up to 5 tubes. Explain the steps in detail on how to perform the serial dilution. Have plenty of sample to work with. Indicate size of pipettes will be using and diluent. Sample is serum. Part B: Give the tube dilution in each tube and the solution dilution in each tube. If the original sample was 550 mg/dL what is the diluted result in tube #4.
Close icon
Play audio
Feedback
Powered by NumerAI
Danielle Fairburn Ivan Kochetkov
Kathleen Carty verified

Jakob Waidelich and 80 other subject Biology educators are ready to help you.

Ask a new question
*

Labs

-

Want to see this concept in action?

NEW

Explore this concept interactively to see how it behaves as you change inputs.

View Labs
*

Key Concepts

-
Key Concept
Premium Feature
Explore the core concept behind this problem.
Play button
Key Concept
Premium Feature
Explore the core concept behind this problem.
Your browser does not support the video tag.
*

Recommended Videos

-
you-are-preparing-a-4-tube-series-of-a-5-fold-serial-dilution-if-tube-1-contains-500-l-of-undiluted-stock-solution-then-how-much-diluent-should-be-added-to-tubes-2-4-2-pt-g33-100-l-250-l-400-18363

You are preparing a 4-tube series of a 5-fold serial dilution. If Tube #1 contains 500 µL of undiluted stock solution, then how much diluent should be added to Tubes #2 - #4? 100 µL 250 µL 400 µL 500 µL You are preparing a 4-tube series of a 5-fold serial dilution. If Tube #1 contains 500 µL of undiluted stock solution, then what volume will be transferred from Tube #1 to Tube #2 to Tube #3 to Tube #4? 500 µL 400 µL 250 µL 100 µL

Jakob W.
how-would-you-set-up-a-series-of-5-tubes-with-a-14-serial-dilution-such-that-you-end-up-with-3-ml-of-solution-in-each-tube-what-is-the-final-dilution-in-each-tube-41606

How would you set up a series of 5 tubes with a 1:4 serial dilution, such that you end up with 3 mL of solution in each tube? What is the final dilution in each tube?

John N.
a-tube-dilution-series-is-performed-on-a-sample-to-check-the-pipetting-skills-of-a-medical-laboratory-technician-student-five-tubes-are-used-in-the-dilution-the-concentration-of-the-sample-i-33578

A tube dilution series is performed on a sample to check the pipetting skills of a medical laboratory technician student. Five tubes are used in the dilution. The concentration of the sample is 1650 mg/dL. The sample is diluted 1/5 (tube 1), rediluted 1/2 (tube 2), diluted again 1/4 (tube 3), then diluted 1/5 (tube 4), and then diluted 1/10 (tube 5). After the student performs the dilution, the diluted specimen will be analyzed, and the student will be graded for precision of pipetting. What will be the diluted concentration in each tube? Please explain step by step how you reached your final answer.

Sri K.
*

Recommended Textbooks

-
Biology for AP Courses

Biology for AP Courses

Julianne Zedalis, John Eggebrecht
achievement 1,496 solutions
Objective Biology for NEET

Objective Biology for NEET

Rajiv Vijay 1st Edition
achievement 1,235 solutions
Introduction to General, Organic and Biochemistry

Introduction to General, Organic and Biochemistry

Frederick A. Bettelheim, William H. Brown, Mary K. Campbell 12th Edition
achievement 1,630 solutions
*

Transcript

-
00:01 All right, so you are preparing a four -tube series of five -fold serial dilution.
00:04 If tube number one contains 500 microliters of undiluted stock solution, and how much dilution should be added to tubes two to four.
00:11 And then you are also preparing a four -tube series of a five -fold serial dilution.
00:16 If tube number one contains 500 micro -liter of undiluted stock solution, then what volume would be transferred from tube one to tube two to two to two -three to two -four? so in a five -fold dilution, the total of, volume of diluted solution should be five times the volume of stock to be used.
00:37 Therefore, in a five -fold dilution of the given stock, we can go as we have 100 micro -leaders of stock plus 400 micro -leaders of diluent...
Need help? Use Ace
Ace is your personal tutor. It breaks down any question with clear steps so you can learn.
Start Using Ace
Ace is your personal tutor for learning
Step-by-step explanations
Instant summaries
Summarize YouTube videos
Understand textbook images or PDFs
Study tools like quizzes and flashcards
Listen to your notes as a podcast
Continue solving this problem
Create a free account to:
  • View full step-by-step solution
  • Ask follow-up questions with Ace AI
  • Save progress and study later
Continue Free
Numerade

Get step-by-step video solution
from top educators

Continue with Clever
or



By creating an account, you agree to the Terms of Service and Privacy Policy
Already have an account? Log In

A free answer
just for you

Watch the video solution with this free unlock.

Numerade

Log in to watch this video
...and 100,000,000 more!


EMAIL

PASSWORD

OR
Continue with Clever