00:01
Okay, so we run a pcr gel and we have essentially four bands that show up on our gel.
00:12
We take this band, cut it out, and then run another pcr using that as a template.
00:19
And as it turns out, we still have these four bands.
00:25
Right.
00:25
So what does that mean in terms of the protein? well, first things first.
00:30
What this means is that the primers.
00:36
Are binding to four different areas.
00:50
That's how we're getting four products.
00:52
More importantly, this top product contains all three of these in it.
01:05
Okay? but because the primers are binding, it tells us really that a, this protein is a tetramer of a single peptide.
01:19
Okay? uh, wait, hold on.
01:25
Yes.
01:25
It's a tetamor of a single peptide.
01:27
So there's four copies...