a. How could the CRISPR/Cas9 system be used to create a translocation between two autosomes in cultured human cells? For an example, you were trying to recreate one of the c-ABL/BCR fusions that is responsible for chronic myeloid leukemia. b. What DNA repair system would your CRISPR experiment depend on for the generation of this translocation? c. If you did manage to generate the c-ABL/BCR fusion in your collection of “CRISPRized” cells, how would you screen for it and determine the junction point between the two genes?
Added by Chavi V.
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The CRISPR/Cas9 system could be used to create a translocation between two autosomes in cultured human cells by introducing double-strand breaks (DSBs) at specific locations in the genome. In this case, you would design guide RNAs (gRNAs) that target the c-ABL Show more…
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