A lead compound containing a free base amine is dissolved in DMSO and tested in phosphate buffer saline for biological activity
Added by John O.
Step 1
Ensure that the compound is fully dissolved by gently stirring or vortexing. Show more…
Show all steps
Your feedback will help us improve your experience
Adi S and 96 other Chemistry 101 educators are ready to help you.
Ask a new question
Labs
Want to see this concept in action?
Explore this concept interactively to see how it behaves as you change inputs.
Key Concepts
Recommended Videos
Q1: Calculate how much DMSO you will need to add to the tubes to bring the concentration to 100 nM. PC-009 (test compound): molecular weight of 392.41 g/mol and there is a total amount of 6.7 mg in the tube. PC-012 (test compound): molecular weight of 496.35 g/mol and there is a total amount of 7 mg in the tube. PC-013 (test compound): molecular weight of 445.51 g/mol and there is a total amount of 7.9 mg in the tube. Q2: Once you bring the concentration to 100 mM, you will then set up a plate where you test at a final concentration of 100 uM, 50 uM, 10 uM, and 5 uM. So you will also need to figure out how much of the 100 mM stock you will need to add to the wells in your plate for each of those concentrations.
Adi S.
what is compound B?
George B.
A drug contains an ionized carboxylate group and shows good activity against its target in in vitro tests. When in vivo tests were carried out, the drug showed poor activity when it was administered orally, but good activity when it was administered by intravenous injection. The same drug was converted to an ester, but proved inactive in vitro. Despite that, it proved to be active in vivo when it was administered orally. Explain these observations.
Sri K.
Recommended Textbooks
Chemistry: Structure and Properties
Chemistry The Central Science
Chemistry
Transcript
Watch the video solution with this free unlock.
EMAIL
PASSWORD