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Q1: Calculate how much DMSO you will need to add to the tubes to bring the concentration to 100 nM. PC-009 (test compound): molecular weight of 392.41 g/mol and there is a total amount of 6.7 mg in the tube. PC-012 (test compound): molecular weight of 496.35 g/mol and there is a total amount of 7 mg in the tube. PC-013 (test compound): molecular weight of 445.51 g/mol and there is a total amount of 7.9 mg in the tube. Q2: Once you bring the concentration to 100 mM, you will then set up a plate where you test at a final concentration of 100 uM, 50 uM, 10 uM, and 5 uM. So you will also need to figure out how much of the 100 mM stock you will need to add to the wells in your plate for each of those concentrations.

          Q1: Calculate how much DMSO you will need to add to the tubes to bring the concentration to 100 nM.
PC-009 (test compound): molecular weight of 392.41 g/mol and there is a total amount of 6.7 mg in the tube.
PC-012 (test compound): molecular weight of 496.35 g/mol and there is a total amount of 7 mg in the tube.
PC-013 (test compound): molecular weight of 445.51 g/mol and there is a total amount of 7.9 mg in the tube.
Q2: Once you bring the concentration to 100 mM, you will then set up a plate where you test at a final concentration of 100 uM, 50 uM, 10 uM, and 5 uM. So you will also need to figure out how much of the 100 mM stock you will need to add to the wells in your plate for each of those concentrations.

Added by Trevor S.

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