Question

A mixture of Alanine (pl 6.02), Arginine (pl 10.76), Aspartic Acid (pl 2.98), Histidine (pl 7.59) and Phenylalanine (pl 5.48) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2? Prompts 1 First ② Second 3 Third 4 Fourth 5 Fifth Answers Select match Select match Select match Select match Select match

Name: a mixture of alanine pl 602 arginine pl 1076 aspartic acid pl 298 histidine pl 759 and phenylalanine pl 548 are separated by cation exchange chromatography what is the order of elution of th 41563
Uploaded: 2022-04-19T03:09:43-08:00
Duration: 1 min 25 s
Channel: Sri K
Description: a mixture of alanine pl 602 arginine pl 1076 aspartic acid pl 298 histidine pl 759 and phenylalanine pl 548 are separated by cation exchange chromatography what is the order of elution of th 41563

          A mixture of Alanine (pl 6.02), Arginine (pl 10.76), Aspartic Acid (pl 2.98), Histidine (pl 7.59) and Phenylalanine (pl 5.48) are separated
by cation exchange chromatography.
What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2?
Prompts
1 First
② Second
3 Third
4 Fourth
5 Fifth
Answers
Select match
Select match
Select match
Select match
Select match

a mixture of alanine pl 602 arginine pl 1076 aspartic acid pl 298 histidine pl 759 and phenylalanine pl 548 are separated by cation exchange chromatography what is the order of elution of th 41563

Added by Jessica S.

Chemistry: Structure and Properties

Nivaldo Tro 2nd Edition

Instant Answer

Solved by Expert Sri K

04/19/2022

Step 1

The more positive the charge, the stronger the binding to the resin, and the later the elution. The pH gradient from 10 to 2 will affect the charge of the amino acids. Show more…

Show all steps

Thanks for your feedback!

A mixture of Alanine (pl 6.02), Arginine (pl 10.76), Aspartic Acid (pl 2.98), Histidine (pl 7.59) and Phenylalanine (pl 5.48) are separated by cation exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2? Prompts 1 First ② Second 3 Third 4 Fourth 5 Fifth Answers Select match Select match Select match Select match Select match