A patient visited the clinic complaining of a skin infection. Gram-positive cocci in clusters were isolated. Which of the following biochemical tests are the main tests used to determine the species of the causative agent for this infection? Select one: a. Catalase and oxidase b. Coagulase and catalase c. Oxidase and coagulase
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Step 1: The presence of Gram-positive cocci in clusters suggests that the causative agent may be Staphylococcus species. Show more…
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A clinical microbiologist wishes to identify two bacterial species from a mixed bacterial skin infection. The microbiologist performed a Gram stain reaction of the sample, and noted by microscopic observation that the sample contains gram-positive cocci in clustered or paired formation and randomly scattered gram-negative bacilli (rods). The Gram stain results are shown in Figure 1. Using the Photographic Atlas and the textbook, describe the steps the microbiologist will use to a) separate the two organisms, and b) confirm their identity. For each step, explain the rationale for your choices. Hint: The microbiologist has access to selective and differential media as well as biochemical tests.
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49. When tested for Catalase activity, the Streptococcal isolate should exhibit positive for catalase activity, negative for catalase activity. Test for catalase activity is of no value in streptococcal identification. Oxidase activity should be tested instead. 50. Which of the following is correct? Streptococcus pyogenes is classified as group A beta hemolytic. Streptococcus pyogenes is classified as group B beta hemolytic. Streptococcus pyogenes will test positive for CAMP factor. Streptococcus pyogenes is resistant to bacitracin. All the above are correct except for (b).
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Staphylococcus aureus is most often associated with being a. coagulase-positive. b. coagulase-negative. c. catalase-negative. d. gram-negative
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