A pH gradient is formed during isoelectric focusing. Which of the following describes how protein molecules distribute themselves during isoelectric focusing? More than one option is correct. Question 5 options: 1. proteins migrate towards the positive electrode at positive pH. 2. proteins migrate in an electric field based on their net charge. 3. proteins cease migration when they have an equal number of positive and negative charges at the ambient pH. 4. proteins migrate in an electric field until pH extremes denature their secondary structure. 5. the isoelectric point of a protein reflects its amino acid composition.
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Step 1: Proteins migrate in an electric field based on their net charge. Show more…
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A pH gradient is formed during isoelectric focusing. Which of the following describes how protein molecules distribute themselves during isoelectric focusing? More than one option is correct. proteins migrate towards the positive electrode at positive pH. proteins migrate in an electric field based on their net charge. proteins cease migration when they have an equal number of positive and negative charges at the ambient pH. proteins migrate in an electric field until pH extremes denature their secondary structure. the isoelectric point of a protein reflects its amino acid composition.
Adi S.
Select all that apply. In isoelectric focusing: a. the isoelectric point (pI) of a protein is the pH at which its net charge is zero. b. the pH of a gel depends on the isoelectric point of the protein. c. a gel for isoelectric focusing contains SDS. d. proteins migrate to the location at which they have no net charge. e. a mixture of proteins with the same isoelectric point is subjected to electrophoresis.
Shaiju T.
Following are the properties of proteins at isoelectric pH (pI) except: a. Proteins will have maximum solubility at pI b. Protein possess equal number of positive and negative charges c Proteins exist in zwitter ionic form d. Below the isoelectric point, they possess net positive charge A B C D
Shalini T.
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