Question

A protein biochemist attempted to determine the amino acid sequence of a decapeptide. Use the results from the trypsin, chymotrypsin, and cyanogen bromide treatments to suggest the amino acid sequence of this decapeptide. Trypsin digestion gave two fragments with multiple residues (not in order): T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr T2: Lys, Met, Val Chymotrypsin digestion gave four fragments with multiple residues (not in order): CT1: Ala, Phe CT2: Thr, Trp CT3: Lys, Met, Tyr, Val CT4: Arg, Gly Treatment with cyanogen bromide yielded a single amino acid, methionine, and a nonapeptide. What is a possible sequence of the decapeptide? Use three‑letter abbreviations in your answer, and add a dash between each residue.

          A protein biochemist attempted to determine the amino acid
sequence of a decapeptide. Use the results from the trypsin,
chymotrypsin, and cyanogen bromide treatments to suggest the amino
acid sequence of this decapeptide.
Trypsin digestion gave two fragments with multiple residues (not
in order):
T1: Ala, Arg, Phe, Gly, Thr, Trp, Tyr
T2: Lys, Met, Val
Chymotrypsin digestion gave four fragments with multiple
residues (not in order):
CT1: Ala, Phe
CT2: Thr, Trp
CT3: Lys, Met, Tyr, Val
CT4: Arg, Gly
Treatment with cyanogen bromide yielded a single amino acid,
methionine, and a nonapeptide.
What is a possible sequence of the decapeptide? Use three‑letter
abbreviations in your answer, and add a dash between
each residue.

Added by Soledad W.

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