1. A sample of an unknown peptide was divided into two aliquots. One aliquot was treated with trypsin, and the other with cyanogen bromide. Given the following sequences of the resulting fragments, deduce the sequence of the original peptide. Trypsin treatment: Asn-Thr-Trp-Met-Ile-Lys Gly-Tyr-Met-Gln-Phe Val-Leu-Gly-Met-Ser-Arg Cyanogen Bromide treatment: Gln-Phe Ile-Lys-Gly-Tyr-Met Ser-Arg-Asn-Thr-Trp-Met
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A sample of an unknown peptide was divided into two aliquots. One aliquot was treated with trypsin; the other was treated with cyanogen bromide. Given the following sequences (N-terminal to C-terminal) of the resulting fragments, deduce the sequence of the original peptide.
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Sample of peptide of unknown sequence was treated with trypsin; another sample of the same peptide was treated with chymotrypsin. The sequences of the smaller peptides are as follows. Deduce the sequence of the original peptide: Trypsin treatment: Met-Val-Ser-Thr-Lys-Val-Ile-Trp-Thr-Leu-Met-Ile-Leu-Phe-Asn-Glu-Ser-Arg Chymotrypsin treatment: Asn-Glu-Ser-Arg-Val-Ile-Trp-Thr-Leu-Met-Ile-Met-Val-Ser-Thr-Lys-Leu-Phe
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Protein sequencing $2 .$ Determine the sequence of a peptide consisting of 14 amino acids on the basis of the following data. Amino acid composition: $(4 S, 2 L, F, G, I, K, M, T, W, Y)$ N-terminal analysis: $\mathrm{S}$ Carboxypeptidase digestion: $L$ Trypsin digestion: $(3 S, 2 L, F, I, M, T, W)(G, K, S, Y)$ Chymotrypsin digestion: $(\mathrm{F}, \mathrm{I}, \mathrm{S})(\mathrm{G}, \mathrm{K}, \mathrm{L})$ (L,S) $(\mathrm{M}, \mathrm{T})$ $(\mathrm{S}, \mathrm{W})(\mathrm{S}, \mathrm{Y})$ N-terminal analysis of $(\mathrm{F}, \mathrm{I}, \mathrm{S})$ peptide: $\mathrm{S}$ Cyanogen bromide treatment: $\left(2 \mathrm{S}, \mathrm{F}, \mathrm{G}, \mathrm{I}, \mathrm{K}, \mathrm{L}, \mathrm{M}^{*}, \mathrm{T}, \mathrm{Y}\right)$ $(2 \mathrm{S}, \mathrm{L}, \mathrm{W})$ $\mathrm{M}^{*},$ methionine detected as homoserine
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