A second patient enters the emergency room at Virtua Memorial. They are complaining of severe diarrhea, vomiting and a fever. They tell you that they have recently been eating an abundance of hamburgers from the Topps meat company. You immediately suspect that the patient is infected with a pathogenic strain of Escherichia coli. In order to confirm this bacterial infection, you take a specimen from the patient in order to perform several tests. Describe would you expect to see for the following tests. You stain the bacteria with methylene blue. What do you expect to see under the microscope? a. Blue cocci b. Blue Bacilli c. Blue spirochetes d. Nothing. Methylene blue is an acidic dy
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coli). E. coli is a type of bacteria that is rod-shaped (bacilli) and is commonly associated with gastrointestinal infections, particularly those linked to undercooked or contaminated meat. Show more…
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A second patient enters the emergency room at Virtua Memorial. They are complaining of severe diarrhea, vomiting, and fever. They tell you that they have recently been eating an abundance of hamburgers from the Topps meat company. You immediately suspect that the patient is infected with a pathogenic strain of Escherichia coli. In order to confirm this bacterial infection, you take a specimen from the patient to perform several tests. Describe what you would expect to see for the following tests. You do a simple stain and stain the bacteria with methylene blue. Do you expect the bacterial cells to be stained with this dye? Why or why not? What shape would the bacteria be if they are indeed Escherichia coli when viewed under a microscope? You decide to perform a gram stain on the bacteria. What would you expect to see if your culture was indeed positive for Escherichia coli? Would the culture be gram-positive or gram-negative? In order to confirm your results from the gram stain, you decide to check for the growth of the microbe on eosin methylene blue agar plates.
Sri K.
You make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use correct aseptic technique by not sterilizing the loop before picking up the bacterial sample for the smear, what results may be seen under the microscope? A Both blue and purple cells may be seen on the slide. B No cells may be visible on the slide. C There may be cells of different shapes, sizes, and arrangements.
Rupsa S.
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