Question

a). Drawing 1: Using nucleotides, make a single-stranded DNA molecule of any sequence, 30 nucleotides long. Use an equal number of A,C, G, and T's. Each nucleotide should be their own color and have an orientation (5' and 3' should be clearly labeled)i). Make the molecule double-stranded b) Drawing 2: Separate the strands for about 7 nucleotides at one end of DNA (you are acting as the helicase), add a primer (3 grey nucleotides) to each side at the replication fork (You are acting as primase). Be sure to make the primers in the correct 5' to 3' direction- label it a. Identify the leading and lagging strand c)Drawing 3: Fill in the nucleotides in, extending both the leading and the lagging strand with colored nucleotides to the end of the exposed nucleotides. d)Drawing 4: Separate the strands farther, moving the replication fork about 8 nucleotides.a. Fill in the nucleotides in, extending the leading strand with colored nucleotide to within 2 nucleotides of the replication fork e)Drawing 5: Add a new primer (3 grey nucleotides) to the lagging strand (recognize why you need to add a primer) at the fork. Extend the lagging strand with colored nucleotides until you reach the earlier primer. Do not connect the colored nucleotide to the grey nucleotide in the earlier primer. f)Drawing 6: Open the replication fork again and then list how you will repeat steps above. g)Drawing 7: Finish separating the strands and continue as above until the two strands are replicated. (do not remove primers or ligate nucleotides- yet h)Drawing 8: Remove primers, fill in with correct nucleotides. Show how you will connect the colored nucleotide with the earlier colored nucleotide to (this is the ligase activity).a. Notice that the replicated strands are identical i). Tell me what is happening to the un-replicated nucleotides at the end of the strands. What does this mean for the cell each time it replicates? For the organism?

          a). Drawing 1: Using nucleotides, make a single-stranded DNA molecule of any sequence, 30 nucleotides long. Use an equal number of A,C, G, and T's. Each nucleotide should be their own color and have an orientation (5' and 3' should be clearly labeled)i). Make the molecule double-stranded

b) Drawing 2: Separate the strands for about 7 nucleotides at one end of DNA (you are acting as the helicase), add a primer (3 grey nucleotides) to each side at the replication fork (You are acting as primase). Be sure to make the primers in the correct 5' to 3' direction- label it a. Identify the leading and lagging strand

c)Drawing 3: Fill in the nucleotides in, extending both the leading and the lagging strand with colored nucleotides to the end of the exposed nucleotides.

d)Drawing 4: Separate the strands farther, moving the replication fork about 8 nucleotides.a. Fill in the nucleotides in, extending the leading strand with colored nucleotide to within 2 nucleotides of the replication fork

e)Drawing 5: Add a new primer (3 grey nucleotides) to the lagging strand (recognize why you need to add a primer) at the fork. Extend the lagging strand with colored nucleotides until you reach the earlier primer. Do not connect the colored nucleotide to the grey nucleotide in the earlier primer.

f)Drawing 6: Open the replication fork again and then list how you will repeat steps above.

g)Drawing 7: Finish separating the strands and continue as above until the two strands are replicated. (do not remove primers or ligate nucleotides- yet

h)Drawing 8: Remove primers, fill in with correct nucleotides. Show how you will connect the colored nucleotide with the earlier colored nucleotide to (this is the ligase activity).a. Notice that the replicated strands are identical

i). Tell me what is happening to the un-replicated nucleotides at the end of the strands. What does this mean for the cell each time it replicates? For the organism?

a). Drawing 1: Using nucleotides, make a single-stranded DNA molecule of any sequence, 30 nucleotides long. Use an equal number of A,C, G, and T's. Each nucleotide should be their own color and have an orientation (5' and 3' should be clearly labeled)i). Make the molecule double-stranded

b) Drawing 2: Separate the strands for about 7 nucleotides at one end of DNA (you are acting as the helicase), add a primer (3 grey nucleotides) to each side at the replication fork (You are acting as primase). Be sure to make the primers in the correct 5' to 3' direction- label it a. Identify the leading and lagging strand

c)Drawing 3: Fill in the nucleotides in, extending both the leading and the lagging strand with colored nucleotides to the end of the exposed nucleotides.

d)Drawing 4: Separate the strands farther, moving the replication fork about 8 nucleotides.a. Fill in the nucleotides in, extending the leading strand with colored nucleotide to within 2 nucleotides of the replication fork

e)Drawing 5: Add a new primer (3 grey nucleotides) to the lagging strand (recognize why you need to add a primer) at the fork. Extend the lagging strand with colored nucleotides until you reach the earlier primer. Do not connect the colored nucleotide to the grey nucleotide in the earlier primer.

f)Drawing 6: Open the replication fork again and then list how you will repeat steps above.

g)Drawing 7: Finish separating the strands and continue as above until the two strands are replicated. (do not remove primers or ligate nucleotides- yet

h)Drawing 8: Remove primers, fill in with correct nucleotides. Show how you will connect the colored nucleotide with the earlier colored nucleotide to (this is the ligase activity).a. Notice that the replicated strands are identical

i). Tell me what is happening to the un-replicated nucleotides at the end of the strands. What does this mean for the cell each time it replicates? For the organism?

Added by Mariano B.

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