Absorbance (412nm): 0.069, 0.113, 0.201, 0.377, 0.730
Concentration of standard solution (µg/mL)
You wish to determine the concentration of hemoglobin in a blood sample by spectrophotometry. You first create a standard curve of the absorbance at 412 nm of several solutions of known hemoglobin concentrations. The data for the standard curve is shown above. What is the concentration (in µg/mL) of hemoglobin in your sample if the absorbance obtained at 412 nm was 0.303?