00:01
In this part, the question is all about the access of the ground -staining procedure simulation with the following link given in the question.
00:09
Take note here that the most important of four -step is basically the decolorization.
00:18
So we have the decolorization step.
00:23
As only this step differentiate between the gram -positive, the gram -positive, and also the negative bacteria.
00:39
If this step, which is the primary dye that stains the lipid layer, for example, we have the peptidoglycan in gram -negative bacteria, which are it destined, that would be lipidocin.
01:04
Lipids are soluble in alcohol and the failure to and the failure to take failure to take up take up the counter stain as alcohol is decolorizer if too much alcohol is used the decolorization may start taking place even in the gram positive bacteria and we may get inaccurate results.
01:43
On the contrary, when less alcohol, so when there is a less alcohol is used, so then there will be a proper disdaining process in gram -negative bacteria.
02:09
And it isn't achieved, and even this might lead to improper, results.
02:15
If application of counterstain, for example, the counter the counter stain is saffronine.
02:22
So we have the saffronine is left out the whole gram staining technique and it would be of no importance in differentiating the positive and also the negative bacteria.
02:36
So therefore here we have the final conclusion for the gram stain.
02:46
So first one is we have the gram stain, gram stain, that would be the primary stain...