Actin filaments have polarity. This means that the two ends can be identified due to structural differences. The plus end is the end to which subunits are added more rapidly, or the end of polymerization. Which of the following would enable you to identify the plus end of actin filaments? Determining the ionic charge of the ends of the actin filaments, adding radiolabeled actin subunits to a mixture of actin filaments in which conditions are favorable for polymerization, or adding radiolabeled actin subunits to a mixture of actin filaments in which conditions favor depolymerization.
Added by Tracy W.
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Determining the ionic charge of the ends of the actin filaments: This option does not provide information about the rate of subunit addition or polymerization, so it would not help identify the plus end. Show more…
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