Adding 10.0 mL of Yeast Culture to 90.0 mL of Water, yields a final Dilution Factor of:
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Total volume = 10.0 mL + 90.0 mL = 100.0 mL Show more…
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A yeast strain is diluted by adding 100 microliters of the culture to 9.9 ml of water, then taking 2 ml of the first dilution and adding 8 ml of water, then taking 250 microliters of the second dilution and adding it to 1.25 ml of water. Finally 100 microliters of the last dilution is plated and 750 colonies grew on the plate. What is the concentration of cells in the original yeast culture? Draw a diagram to show how you arrived at your answer.
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What is the dilution of a sample when 10 ml of the original sample has been added to 90 ml of culture? Please show your working below.
Calculate the final dilution factor (FDF) in the following scenario: Dilution 1: 1 ml sample Ă 99 ml water, Dilution 2: 100 ul Dil 1 Ă 900 ul water, Dilution 3: 100 ul Ă 900 ul water, plate 100 ul.
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