an average count of 42 cellsmm2 grid is counted in a hemocytometer for a 25 ml suspension of cel

total number of cells that is equals to number of average counted cell multiply by dilution vect

Hello everyone for this question we have to answer the various parts starting with first where w

The question that is given over here, it says that we have cells that are suspended in 6 ml of c

In cell culture, some cell lines need to be seeded with a particular seeding density to have cel

For number one, the correct answer would be 1 .23 times 10 raised to the power of 6 viable cells

Question

An average count of 42 cells/mmÂ² grid is counted in a hemocytometer for a 2.5 ml suspension of cells. 1. What is the cell concentration of the suspension? 2. Approximately how many cells are in the entire sample? Show your calculation. 3. 8 dishes of cells at 1.2 x 10^5 cells/dish are needed for an experiment. How many ml of the suspension will be needed for the experiment? Are there enough cells? Show your calculation. 4. How many cells would have to be actually added to a plate (this is usually called "seed") to begin a culture with 3.5 x 10^5 cells/dish, if the line of cells is known to have a plating efficiency of 65%? Show your calculation. *Plating efficiency refers to the % of cells that can survive after being seeded in a culture container.

          An average count of 42 cells/mmÂ² grid is counted in a hemocytometer for a 2.5 ml suspension of cells.

1. What is the cell concentration of the suspension?
2. Approximately how many cells are in the entire sample? Show your calculation.
3. 8 dishes of cells at 1.2 x 10^5 cells/dish are needed for an experiment. How many ml of the suspension will be needed for the experiment? Are there enough cells? Show your calculation.
4. How many cells would have to be actually added to a plate (this is usually called "seed") to begin a culture with 3.5 x 10^5 cells/dish, if the line of cells is known to have a plating efficiency of 65%? Show your calculation. *Plating efficiency refers to the % of cells that can survive after being seeded in a culture container.

Added by Mariano M.

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