Question

You have cells suspended in 6 ml of culture medium at 4.7x10^6 cells/ml. You plan to seed three 96-well plates for CPE assays. You plan to seed 5x10^4 cells per well and want to add 150 µl of cell suspension per well. 1. How many total ml of cell suspension will you need for seeding 3 plates? 2. Always prepare slightly more total ml of cell suspension than you will need. How much will you prepare? (A general rule is 10% extra) 3. At what concentration do you need to prepare your cell suspension for seeding? (In other words, how many cells/ml do you need in order to have the right amount of cells in only 150 µl?) 4. How will you prepare the cell suspension? (how many ml of your current suspension will you add to how many ml of culture medium?)

          You have cells suspended in 6 ml of culture medium at 4.7x10^6 cells/ml. You plan to seed three 96-well plates for CPE assays. You plan to seed 5x10^4 cells per well and want to add 150 µl of cell suspension per well.

1. How many total ml of cell suspension will you need for seeding 3 plates?
2. Always prepare slightly more total ml of cell suspension than you will need. How much will you prepare? (A general rule is 10% extra)
3. At what concentration do you need to prepare your cell suspension for seeding? (In other words, how many cells/ml do you need in order to have the right amount of cells in only 150 µl?)
4. How will you prepare the cell suspension? (how many ml of your current suspension will you add to how many ml of culture medium?)

Added by Timothy O.

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