An enzyme that causes antibiotic resistance to penicillin (give the formal, chemical based name only)
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Step 1: Identify the enzyme responsible for antibiotic resistance to penicillin. Show more…
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Destroying the Trojan horse. Penicillin is hydrolyzed and thereby rendered inactive by penicillinase (also known as $\beta$ -lactamase), an enzyme present in some penicillinresistant bacteria. The mass of this enzyme in Staphylococcus aureus is $29.6 \mathrm{kDa}$. The amount of penicillin hydrolyzed in 1 minute in a $10-\mathrm{ml}$ solution containing $10^{-9} \mathrm{g}$ of purified penicillinase was measured as a function of the concentration of penicillin. Assume that the concentration of penicillin does not change appreciably during the assay. (GRAPHS CANNOT COPY) (a) Plot $V_{0}$ versus $[\mathrm{S}]$ and $1 / V_{0}$ versus $1 /[\mathrm{S}]$ for these data. Does penicillinase appear to obey Michaelis-Menten kinetics? If so, what is the value of $K_{\mathrm{M}}$ ? (b) What is the value of $V_{\max } ?$ (c) What is the turnover number of penicillinase under these experimental conditions? Assume one active site per enzyme molecule.
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