00:01
So an important feature of restriction enzyme is that each enzyme only recognize specific palindrome and cut the dna only at the specific sequence of basis palindromic sequence.
00:14
And then it can be repeated a couple of time on a strand of dna, and then the specific enzyme will only cut the palindromic sequence no matter what species of dna is coming from.
00:27
So now we know if there is a gaa ttc palindrome and it repeated four times on the same piece of linear dna and how many restriction enzyme fragment will be present when the dna is being digested.
00:44
Okay, so let's say we have a double stringent dna and this gaa ttc is being found four times.
00:56
So i'm going to use a bar to represent each gaattc palindromic sequence.
01:05
So you can see every time there is a gaattc sequence, the enzyme is going to cut the dna right here.
01:13
So the linear dna is being cut one, two, three, four times, and it will produce one, two, three, four, five fragments.
01:34
So for any linear dna, it will be cut by the restriction enzyme at the restriction site, and it will produce a five fragments...