Question

An objective of an electron microscope operating in air has a numerical aperture of 0.65. The light used for illumination has a wave length of 530 nm. What is the depth of focus for the objective lens? How flat the specimen should be to obtain the complete surface information using this microscope?

Name: an objective of an electron microscope operating in air has a numerical aperture of 065 the light used for illumination has a wave length of 530 nm what is the depth of focus for the objecti 06257
Uploaded: 2021-11-28T06:44:47-08:00
Duration: 5 min 54 s
Channel: Ameer Said
Description: an objective of an electron microscope operating in air has a numerical aperture of 065 the light used for illumination has a wave length of 530 nm what is the depth of focus for the objecti 06257

          An objective of an electron microscope operating in air has a numerical aperture 
of 0.65. The light used for illumination has a wave length of 530 nm. What is the 
depth of focus for the objective lens? How flat the specimen should be to obtain 
the complete surface information using this microscope?

Added by Rita H.

University Physics with Modern Physics

Hugh D. Young 14th Edition

Instant Answer

Solved by Expert Ameer Said

11/28/2021

Step 1

61 * λ) / (NA)^2 where: λ = wavelength of light = 530 nm = 530 x 10^-9 m NA = numerical aperture = 0.65 Depth of focus = ±(0.61 * 530 x 10^-9) / (0.65)^2 Depth of focus = ±(0.61 * 530 x 10^-9) / 0.4225 Depth of focus = ±(0.000000000318) / 0.4225 Depth of focus = Show more…

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An objective of an electron microscope operating in air has a numerical aperture of 0.65. The light used for illumination has a wave length of 530 nm. What is the depth of focus for the objective lens? How flat the specimen should be to obtain the complete surface information using this microscope?