An unknown organism has the following differential test results: Catalase positive (+) Motility positive (M+) Mannitol fermentation negative (A-) Casein hydrolysis positive (+)/Citrate utilization positive (+) Lysine decarboxylase negative (-) Based on the dichotomous key above, the scientific name of the organism is Blank 1. Blank 1
Added by Gregorio C.
Step 1
Step 1: Identify the organism as catalase positive (+), so it belongs to the group of catalase positive bacteria. Show more…
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Unknown bacteria identification. Find genus and species of unknown bacteria. You can use at least 3 species to narrow it down. Given Information: Cell size - 0.8 * 2.7 micrometers Cell shape - straight rod Cell arrangement - chains, pairs, single cells Gram reaction - Purple (gram positive) Motility - Brownian motion only Endospore - stains pink Inclusion bodies - Absent Sporangium - diameter of spore is equal to a vegetative cell or absent Water-soluble pigments - No Colony pigmentation - No pigment (White or cream) Odor from the plate - none identified Oxidative/fermentation glucose with oil - yellow color Oxidative/fermentation glucose without oil - yellow color Temperature growth range - 30-37 degrees Celsius Thioglycolate broth tube - growth on the top and growth on the bottom MacConkey agar - no growth Glucose utilization - positive Sucrose - positive Maltose - negative Xylose - negative Mannitol - positive Lactose - positive Hemolysis - agar is weakly greening around colonies (alpha hemolysis) Oxidase - brown color on the filter (negative) Catalase - no bubbles produced (negative) Gelatinase - media is stiff after chilling (negative) Nitratase - media remains colorless (negative for nitrate test) Citrase - slant is green (negative) Find the genus and species.
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Trying to identify my unknown bacteria for microbiology. What is the genus and species of a gram-negative microbe that is oxidase positive and gelatin test negative? Other test results below: Biochemical Test Results Motility Motile FTM Facultative Aerobic Gelatin - Carbohydrate Fermentation Glucose Ferments All Lactose Mannitol Maltose Sucrose Methyl Red Test & Voges-Proskauer Test (MR-VP) Methyl Red: + VP: + Catalase + Oxidase + Nitrate Reductase + Starch Hydrolysis - Casein Hydrolysis (Skim Milk) - Fat Hydrolysis - Tryptophan Hydrolysis Indole - Urease Test + but slow Hydrogen Sulfide Production - Citrate Utilization - Phenylalanine Deamination - Litmus Milk Pink (acid reaction, lactose fermented) Lysine Decarboxylase +
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Unknown 1: Gram stain: Gram-negative rods (GNR) Oxidase: Positive TSI: K/K Nitrate: Negative Gelatinase: Positive Lysine decarboxylase: Positive Motility: Present Urea: Negative Citrate: Positive Pigment: Yellow Unknown 2: Gram stain: Gram-negative rods (GNR) Oxidase: Negative TSI: K/K ONPG: Positive Lysine decarboxylase: Positive Esculin: Positive Citrate: Positive Urea: Negative PAD: Negative Indole: Negative VP: Negative Gelatinase: Positive
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